brite cell line—gl261 red-fluc  (BioWare Corporation)

Journal: Cell Reports Medicine

Article Title: PPRX-1701, a nanoparticle formulation of 6′-bromoindirubin acetoxime, improves delivery and shows efficacy in preclinical GBM models

doi: 10.1016/j.xcrm.2023.101019

Figure Lengend Snippet:

Article Snippet: Mouse: GL261fluc2 , PerkinElmer , BW134246.

Techniques: Recombinant, Protease Inhibitor, Plasmid Preparation, Staining, cDNA Synthesis, SYBR Green Assay, Cell Isolation, Microarray, Software, Imaging, Light Microscopy

Journal: Yonsei Medical Journal

Article Title: Selective Inhibition of PI3K Isoforms in Brain Tumors Suppresses Tumor Growth by Increasing Radiosensitivity

doi: 10.3349/ymj.2022.0414

Figure Lengend Snippet: Combination effect of irradiation and PI3K isoform inhibitors in the LN229 human glioma cell line. (A) Clonogenic survival curves: LN229 were treated with PI3K isoform-selective inhibitor at 1 h after 2 Gy irradiation. (B) Expression of phosphor-γ-H2AX in GL261 cells after a combination of radiation and PI3K isoform inhibitors ( p : CON vs. IR+GDC0941; *** , CON vs. IR+GDC0032; *** ). Treatment doses were GDC0941 0.1 µM, GDC0032 0.1 µM, CAL101 1 µM, and IPI145 1 µMs. Statistical significance was set at p <0.05. *** p <0.001. PI3K, phosphoinositide 3-kinase; AKT, protein kinase B.

Article Snippet: The mouse-derived cell line GL261- luc (purchased from PerkinElmer, Waltham, MA, USA) and human-derived cell line LN229 (purchased from ATCC, Manassas, VA, USA) were used in the present study.

Techniques: Irradiation, Expressing

Journal: Yonsei Medical Journal

Article Title: Selective Inhibition of PI3K Isoforms in Brain Tumors Suppresses Tumor Growth by Increasing Radiosensitivity

doi: 10.3349/ymj.2022.0414

Figure Lengend Snippet: Effect of irradiation and PI3K isoform inhibitors in the GL261 mouse glioma cell line. (A) Dose versus clonogenic survival curves and immunofluorescence images of phosphor-γ-H2AX. GL261- luc cells were treated with PI3K isoform-selective inhibitors for 24 h (doses ranging from 0.1 to 10 µM). Data are expressed as the percentage of (B) MTT cell proliferation assay. (C) Protein expression of AKT activation in GL261- luc . Statistical significance was set at p <0.05. ** p <0.01; *** p <0.001. PI3K, phosphoinositide 3-kinase; AKT, protein kinase B.

Article Snippet: The mouse-derived cell line GL261- luc (purchased from PerkinElmer, Waltham, MA, USA) and human-derived cell line LN229 (purchased from ATCC, Manassas, VA, USA) were used in the present study.

Techniques: Irradiation, Immunofluorescence, MTT Cell Proliferation, Expressing, Activation Assay

Journal: Yonsei Medical Journal

Article Title: Selective Inhibition of PI3K Isoforms in Brain Tumors Suppresses Tumor Growth by Increasing Radiosensitivity

doi: 10.3349/ymj.2022.0414

Figure Lengend Snippet: Combination effect of irradiation and PI3K isoform inhibitors in the GL261 mouse glioma cell line. (A) Clonogenic survival curves: GL261- luc were treated with PI3K isoform-selective inhibitor at 1 h after 1 Gy irradiation. (B) Expression of phosphor-γ-H2AX in GL261 cells after combination of radiation and PI3K isoform inhibitors (CON vs. IR only; *** , CON vs. IR+GDC0941; *** , CON vs. IR+GDC0032; *** , CON vs. IR+CAL101; *** , CON vs. IR+IPI145; ** ). Treatment doses were GDC0941 0.1 µM, GDC0032 0.1 µM, CAL101 1 µM, and IPI145 0.1 µM. Statistical significance was set at p <0.05. ** p <0.01; *** p <0.001. PI3K, phosphoinositide 3-kinase; AKT, protein kinase B.

Article Snippet: The mouse-derived cell line GL261- luc (purchased from PerkinElmer, Waltham, MA, USA) and human-derived cell line LN229 (purchased from ATCC, Manassas, VA, USA) were used in the present study.

Techniques: Irradiation, Expressing

Journal: Yonsei Medical Journal

Article Title: Selective Inhibition of PI3K Isoforms in Brain Tumors Suppresses Tumor Growth by Increasing Radiosensitivity

doi: 10.3349/ymj.2022.0414

Figure Lengend Snippet: Inhibition of the PI3K-α isoform increases radiosensitivity in brain tumor in vivo. After intracranial implant of GL261- luc cells, 10 Gy irradiation with or without GDC0032 (5 mg/kg). (A) IVIS imaging of mouse brain tumors from within 7–16 days post therapy. (B) Kaplan–Meier survival analysis (p: CON vs. IR only; * , CON vs. IR+GDC0032; ** ). Statistical significance was set at p <0.05. * p <0.05; ** p <0.01. PI3K, phosphoinositide 3-kinase; AKT, protein kinase B.

Article Snippet: The mouse-derived cell line GL261- luc (purchased from PerkinElmer, Waltham, MA, USA) and human-derived cell line LN229 (purchased from ATCC, Manassas, VA, USA) were used in the present study.

Techniques: Inhibition, In Vivo, Irradiation, Imaging

Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

Article Title: Concurrent Dexamethasone Limits the Clinical Benefit of Immune Checkpoint Blockade in Glioblastoma

doi: 10.1158/1078-0432.CCR-20-2291

Figure Lengend Snippet: Kaplan-Meier OS estimates are depicted, with comparison by logrank test and Cox regression. (A) To assess concurrent dexamethasone’s effect on a dose-intensive schedule of anti-PD-1 with or without RT in GL261-luc2 mice (n=8/group from a single experiment), anti-PD-1 was administered IP via a loading dose (500 μg) followed by 5 additional doses (250 μg/dose) at 3-day intervals. RT was administered in 2 Gy fractions/day for 5 days beginning on day 6. Dexamethasone was delivered IP daily from days 6–27 at 10 mg/kg. (B) For GL261-luc2 mice (n=8/group from a single experiment), anti-PD-1 (αPD1) was administered IP via an abbreviated dosing schedule every 3 days beginning on day 6 for a total of 4 doses (250 μg/dose). (C) For CT-2A-luc mice (n=8–16/group, derived from two experiments), anti-PD-1 was administered IP via a loading dose (500 μg) followed by 7 additional doses (250 μg/dose) at 3-day intervals. RT was administered in 2 Gy fractions/day for 5 days beginning on day 6. Dexamethasone was delivered IP daily from days 6–27 at 10 mg/kg.

Article Snippet: Cell Lines, Antibodies, and Reagents Luciferase-transduced GL261 cells (GL261-luc2; PerkinElmer, Inc., Waltham, MA) were expanded and frozen at the same generation.

Techniques: Comparison, Derivative Assay